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: Researchers from Brown University and Ladies & Infants Hospital of Rhode Island, have found a way to provide information on gene expression from fertile eggs, they developed without violating extract. Need supplies "polar" in the cells of the same information as the eggs themselves, the researchers reflect the challenges of in vitro fertilization, prospective parents and their doctors the best information they have about the eggs that they can get the extract determined.Given to fertilize and implant testing. A new study from Brown University and Females & Infants Hospital of Rhode Island has found a way to see which genes of each egg without affecting their expression is found. As researchers learn more about how these genes affect the development of the embryo, the new technique could ultimately parents and physicians with an overview of the eggs may contribute to more appropriate embryos.In research, now in the press of the Journal of Biological Chemistry was the team of doctors and biologists are able to sequence the genetic material or mRNA transcribed in oocytes and to a large scientific pinched first of these small structures as a "polar body". By comparing the gene sequences in polar bodies and their host eggs, the researchers determined that the polar bodies are a true reflection of the activity of the egg "Genetics may offer." We can now use the polar body biopsy is a cytoplasmic natural, "said co-author Sandra Carson, professor of obstetrics and gynecology at the Warren Alpert Medical School of Brown University and director of the Center for Reproduction and Infertility ladies and infants are the body Hospital.Polar dispense with the second egg in which copies of the chromosomes, the germ cells do not need it. But the polar bodies also capture a microcosm of the mRNA of the egg, the genetic material, genes were transcribed and if a cell is attached to proteins on the response to genetic instructions.
Upcoming events generated in the last two years to find the team for the first time the mRNA in polar bodies. Now they have the transcribed into 22 pairs of human eggs and polar bodies, and confirmed that, which is in the polar body is a good indicator of what is in how little eggs.Given mRNA in organisms polar, the task was not easy, said Gary Wessel, professor of biology, but by a clever combination of amplification techniques and analysis by the senior author and students Adrian Empire and the second author Peter Klatsky, the The team has done. "There is no reason it should work health, just because there is so little material," Wessel said. "Single-cell sequencing is a major challenge. "Cover your paris, the team analyzed the majority of samples into two pools of 10 cells each, for example, comparison of mRNA in 10 eggs with mRNA in the 10 related polar body. But to their surprise, they were also able, resulting in two separate eggs and polar bodies directly.What they found that more than 14,000 genes can be expressed in eggs. Of these, over 90 percent of the genes found in the polar bodies are also eggs and 700 genes most frequently observed in the polar bodies have been identified among the 460 most frequently in eggs.
against clinical use, "It seems the polar body reflects what is happening in the bud, "said Mr. Carson." Because the egg is the main driver of the first three days of human embryonic development, so that we find in the polar body gives us a clue about what has past this time. "But Carson and Wessel acknowledged that more research is needed tool.Finding How to become a clinically useful to create genes that affect the viability of embryos is the next big step. Developed with new knowledge and techniques in their study, researchers, Scientists have been able mRNA polar bodies of eggs that are fertilized and analyzed the progress of the resulting embryo. Once key genes are known, they could quickly search for dosages of these genes in the polar bodies, so that doctors and patients could get the most eggs. A sufficiently advanced technology could be used for selection, the eggs in the bank be used for future use. "We do not know the exact answer, it is these messages to, or necessarily the purpose of the function of the cell, but it's coming, "said Carson. "Now we have the words but not sentences."Health information:
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